Operating procedures and precautions for biosafety cabinets

1
Before operation, all items required for this operation should be moved into the safety cabinet to avoid
Frequently crossing the air curtain with both arms to disrupt the airflow; And use 70% alcohol before moving in
Wipe the surface for disinfection to remove contamination.
2. Turn on the fan for 5-10 minutes and wait for the air inside the cabinet to be purified and the airflow to stabilize
Then proceed with the experimental operation. Slowly insert both arms into the safety cabinet, at least remain still
Stop for 1 minute to stabilize the airflow inside the cabinet before proceeding with the operation.
3. No items unrelated to this experiment shall be placed in the safety cabinet. Placement of items inside the cabinet
The clean area, semi polluted area, and polluted area should be basically separated during operation
Convenient access to items during the journey, and no crossing between the three zones. Items should be relied upon as much as possible
Place it back, but do not block the airway opening to avoid interfering with the normal flow of airflow.
4. The operation should be carried out from the clean area to the contaminated area to avoid crossing
Pollution. To prevent possible splashing droplets, they can be placed on the countertop Apply disinfectant to the upper bunk
A soaked towel or gauze that cannot cover the safety cabinet grille.
5. During the operation inside the cabinet, it is strictly prohibited to use open flames such as alcohol lamps to avoid production
The generated heat generates airflow, which interferes with the stability of the airflow inside the cabinet; And open flames may cause damage
Bad HEPA filter.
6. Try to minimize the movement of people behind you and quickly open and close doors during work,
To prevent unstable airflow inside the safety cabinet.
7. During experimental operations, do not open the glass window and ensure that the operator
The face is above the work window. When operating inside the cabinet, the movements should be gentle and soothing,
6. Try to minimize the movement of people behind you and quickly open and close doors during work,
To prevent unstable airflow inside the safety cabinet.
7. During experimental operations, do not open the glass window and ensure that the operator
The face is above the work window. When operating inside the cabinet, the movements should be gentle and soothing,
Prevent affecting the airflow inside the cabinet.
8. The safety cabinet should be regularly inspected and maintained to ensure its normal operation.
If any abnormal operation of the safety cabinet is found during work, it should be stopped immediately
Take corresponding measures and notify relevant personnel.
9. After the work is complete, close the glass window and keep the fan running for 10 days~
15 minutes, simultaneously turn on the UV light and irradiate for 30 minutes.
10. The safety cabinet should be cleaned and disinfected regularly, and pollutants on the countertop inside the cabinet can be detected
After completing the work and disinfecting with UV light, wipe with 2% 84 disinfectant solution. cabinet
The outer surface should be wiped daily with 1% 84 disinfectant.
Prevent affecting the airflow inside the cabinet.
8. The safety cabinet should be regularly inspected and maintained to ensure its normal operation.
If any abnormal operation of the safety cabinet is found during work, it should be stopped immediately
Take corresponding measures and notify relevant personnel.
9. After the work is complete, close the glass window and keep the fan running for 10 days~
15 minutes, simultaneously turn on the UV light and irradiate for 30 minutes.
10. The safety cabinet should be cleaned and disinfected regularly, and pollutants on the countertop inside the cabinet can be detected
After completing the work and disinfecting with UV light, wipe with 2% 84 disinfectant solution. cabinet
The outer surface should be wiped daily with 1% 84 disinfectant.
11. Items used in the cabinet should be disinfected before being removed to prevent the spread of pathogens
Microorganisms carry out and pollute the environment.
Precautions for Biosafety Cabinets:
Slow movement principle: In order to avoid affecting the normal airflow status, when operating inside the cabinet
Hands should move as gently as possible.,
Principle of parallel placement of items: To avoid cross contamination between items
Elephant production, items placed in the cabinet should be arranged horizontally in a straight line as much as possible to avoid
Cross contamination is caused during the return air process. At the same time, avoid blocking the back return air grille and affecting it
Normal wind path.
Principle of avoiding vibration: Try to avoid vibrating instruments (such as centrifuges, vortices) inside the cabinet as much as possible
The use of oscillators, etc., as vibration can cause particles to accumulate on the filter membrane
The quality is shaken off, resulting in a decrease in the cleanliness of the interior of the operating room. At the same time, if it is on the front operating surface
Balance failure can also cause contamination of the safety cabinet to the operator.
Principle of moving different sample cabinets: When two or more items need to be moved inside the cabinet,
Always follow the principle of moving low polluting items to high polluting items to avoid pollution
Items with high sexual activity can cause extensive pollution inside the cabinet during movement.
Principle of using open flames: Try not to use open flames inside the cabinet. Because it has been used with open flames before
The small particle impurities generated during the process will be carried into the filter membrane area, and these high-temperature impurities
It will damage the filter membrane. When it is unavoidable to use it, it is advisable to use a low flame
The original lamp.