What operating procedures should be noted when using a microbial limit detector

What operating procedures should be noted when using a microbial limit detector

Microbial limit detector is a device specifically designed for microbial detection and counting, widely used in research institutions, inspection and quarantine institutions, quality monitoring institutions, pharmaceutical industry, food industry, and other fields. Its main function is to qualitatively and quantitatively analyze the microorganisms in the sample through specific methods and techniques, thereby ensuring that the quantity of microorganisms in the product or environment meets relevant standards and requirements.
When using a microbial limit detector, it is necessary to pay attention to the following operating procedures:
1. Sample preparation: Firstly, preprocess the sample to be tested, such as dilution, filtration, etc., to ensure the accuracy of the test results. Take samples as required to ensure their representativeness and avoid external contamination. Meanwhile, transfer the sample to a sterile container to avoid cross contamination.
2. Detection operation: Turn on the power of the inspection instrument and ensure that all parameters are displayed normally. Set detection parameters, including sample volume, culture medium type, etc. Under sterile conditions, transfer the sample to the culture medium to ensure uniform distribution of the sample. In addition, close the culture dish to ensure close contact between the dish and the culture medium, and promote the growth of microorganisms. Place the culture dish into the inspection instrument and perform the test according to the requirements of the instrument.
3. Result analysis and recording: Based on the obtained results, conduct data analysis according to relevant standards, and determine whether the sample meets the microbial limit requirements. Record the test results and analysis in relevant data tables or files to ensure data traceability.
4. Daily inspection and maintenance: Before and after using the instrument, operators should ensure that the equipment is clean and sterile. In daily inspections, it is also necessary to pay attention to whether the pressure difference and temperature and humidity in the clean area are within the specified range, and to perform UV disinfection on the surfaces of used instruments and samples. At the same time, regularly clean, calibrate, and maintain the instruments to ensure their accuracy and reliability. In addition, there are some special precautions:
Personnel should undergo training and pass the assessment, possess basic knowledge of microorganisms, and adhere to the code of conduct in clean rooms.
During the inspection, the action amplitude should be small to avoid affecting the airflow direction.
◆ Pay attention that the liquid height of the test solution should not exceed the large mark of the filter cup.
Select the filter membrane material based on the characteristics of the tested product and ensure the integrity of the filter membrane before and after filtration.
When the instrument is not working, please turn off the power.
◆ Do not filter liquids with strong acids, bases, oxidants, or corrosive substances.
When the test sample contains insoluble particles or suspended solids, they should be removed to prevent clogging of the filter membrane.
Before filtration, ensure good sealing of the pipeline.
Following these operating procedures and precautions can ensure the accuracy and reliability of the microbial limit detector, thereby obtaining accurate detection results.