How to properly handle biochemical waste during use of biosafety cabinets?

How to properly handle biochemical waste during use of biosafety cabinets?

Biochemical waste mainly comes from biological experiments, such as cell culture, enzyme experiments, protein purification, genetic engineering, cloning, PCR amplification, enzyme-linked immunosorbent assay (ELISA), etc. These experiments will produce some cell culture waste, petri dishes, centrifuge tubes, pipettes, reagent bottles, tip waste, waste liquid, solid waste, etc.

The biochemical waste generated by the biosafety cabinet during the experiment needs to be handled correctly to protect the laboratory environment and the health and safety of the experimental personnel. Common biochemical wastes include laboratory supplies, media, strains, cells and tissues.

First of all, the biochemical waste needs to be sorted. In general, solid biochemical waste, liquid biochemical waste and chemical waste liquid need to be stored in different containers, and the name and nature of the waste are clearly marked.

Solid biochemical waste, including reagent bottles, pipettes, liquid waste tubes, plastic centrifugal tubes, etc., should be placed directly into specialized biological waste tanks that can be subjected to specialized treatment, such as high temperature autoclave sterilization or chemical treatment.

Liquid biochemical waste needs to be collected in special collection containers, such as polyethylene bottles or other compliant containers, to avoid direct drainage. For some waste fluids that pose a risk of contamination, such as those containing radioactive substances, bacteria and viruses, more stringent treatment methods are required.

Chemical waste liquids need to be collected separately from other waste liquids and stored in compliant containers, preferably stored separately or handed over to specialized institutions for treatment.

The relevant laws and regulations need to be followed when carrying out waste disposal, and the laboratory rubbish bins, biological waste bins and waste liquid collection containers should be cleaned and replaced regularly.