How to choose a carbon dioxide incubator 2/4

2、 The four core hardware standards determine the long-term stability of the device
1. Temperature control system: accuracy baseline ± 0.1 ℃, uniformity cannot be ignored
Cells are extremely sensitive to temperature, and a temperature difference of ± 0.5 ℃ can cause differences in cell proliferation rate. The temperature control accuracy of regular equipment must reach ± 0.1 ℃, while paying attention to the uniformity of temperature throughout the entire area, and not just looking at single point nominal parameters. Poor quality models simplify air ducts and cut corners on fans. The temperature difference between the front and back, top and bottom of the box can reach more than 1 ℃. The experimental results of parallel samples in the same box cannot be repeated.
High quality models use imported silent variable frequency compressors, combined with full range turbulent air ducts and integrated circulating fans, without local airflow directly blowing damage to suspended cells; Equipped with automatic defrosting, door heating and anti condensation structure, double-layer insulated glass door to prevent inner wall dripping from contaminating samples, 7-inch touch screen segmented programming, supporting 0-30 multi-stage cultivation programs, 9999 hour timed operation, multiple sound and light alarms for overheating, power failure, and gas loss, and power-off recovery function to retain experimental data and avoid long-term cultivation failure.
2. Clean inner liner design, reducing the probability of pollution from the source
Mycoplasma and fungal contamination are the most common problems in cell experiments, and the internal structure directly determines the difficulty of cleaning. When making a purchase, priority should be given to choosing a 304 high-precision mirror stainless steel inner liner, with no sharp welds or dirt hiding gaps; Low priced models on the market often use ordinary cold-rolled steel, and the right angle welds are prone to residual culture solution, which can breed mold for a long time.
The whole machine is optimized for waterproof isolation. The circuit board and sensor connectors are all external. The inner tank can be washed directly with clean water. The operation of disinfection and sterilization is simple after the culture solution leaks and a large area of pollution; Built in full range UV sterilization lamp, no disinfection and sterilization blind area, with removable silicone door seals, can be separately disassembled for high-temperature sterilization, taking into account the needs of daily disinfection and sterilization and deep cleaning.
3. Airtightness and humidity control
Poor airtightness of the box will continue to consume CO ₂ steel cylinder gas, increasing long-term consumable costs, while external bacteria continue to invade. The airtightness can be tested by setting 5% CO ₂ at no load and observing the concentration attenuation amplitude after standing for 24 hours. The attenuation value of high-quality equipment is less than 0.2%. Maintain a stable humidity of 90% -95% RH to avoid evaporation and concentration of the culture medium. Prioritize stainless steel integrated humidification water tray, which is corrosion-resistant, easy to clean, and rejects impurities from long-term high humidity aging of plastic water trays.
4. Security protection and intelligent traceability
Compliance laboratories and pharmaceutical companies need complete data traceability functions, and equipment should automatically record temperature and humidity, CO ₂ concentration, and door opening and closing records, support data export and archiving, and meet GLP and GMP audit requirements; Standard password permission lock, automatic power-off due to overheating, gas leakage warning, door control start stop, automatic cut-off of air intake and fan when opening the door, reducing the influx of external air into the polluted box and lowering gas loss.