Use laminar flow cabinet for tissue culture inoculation

Plant cell tissue culture, such as edible mushroom production and eucalyptus seedling inoculation, has high requirements for the production environment. Therefore, in the process of operation and cultivation, the most important thing is to prevent bacterial infection, and sterile equipment is very important. According to foreign experience, plant tissue culture laboratories and production workshops should generally be produced in sterile environments. However, due to the high construction, operation, and maintenance costs of sterile rooms, they are currently not widely popularized in China. The main method currently used in China is local purification, and ultra clean workbenches have become the preferred equipment. Tissue culture ultra clean workbenches are widely used in environments such as plant tissue culture, laboratories, and edible mushroom production. Some edible mushroom tissue culture uses an assembly line inoculation ultra clean table, which is called an assembly line ultra clean table.
The sterile room is mainly used for disinfection, inoculation, and subculture transfer of plant materials. This is the most critical part of plant tissue culture research, which is related to important indicators such as the contamination rate of the culture and the inoculation rate. The ground, ceiling, and walls should be as smooth and dust-free as possible, and easy to take various cleaning and disinfection measures.
The sterile room for inoculation generally has two rooms, inside and outside. The smaller room outside is the preparation room (for operators to change work clothes, work hats, slippers, wash hands, and prepare for external sampling. After changing clothes, washing hands, disinfecting, and entering the air shower room, the dust on the personnel entering the workshop is blown off to prevent employees from bringing hair and other substances into the workshop. Then, the sterile culture materials are sterilized through the transfer window and sent to the inoculation room to prevent the introduction of bacteria, ensuring that the sterile room can conduct sterile operations for a long time and in large quantities). The interior is slightly larger for vaccination purposes.
There should be ultraviolet sterilization lamps or ozone generators installed in the seedless room, and the lights should be turned on for at least 20 minutes before operation. At the same time, indoor spaces should be regularly fumigated with formaldehyde and potassium permanganate vapor (or with 70% alcohol or 0.5% phenol mist for dust reduction and disinfection). The sterile operating room should be equipped with fixed and mobile stages, wide mouthed bottles, alcohol lamps, alcohol swabs, instrument racks, surgical scissors, dissecting knives, dissecting needles, tweezers, etc. for inoculation. In recent years, most laboratories have adopted ultra clean workbenches instead of sterile operating rooms. The ultra clean workbench is not only easy to operate, but also has good results.