Laminar flow cabinet, complete analysis of cleaning and sterilization strategies 2

1） Operating with UV light on, sterilizing while illuminating "- completely wrong!
The effective sterilization range of ultraviolet lamps is still air. When turned on, operation can expose the human body to ultraviolet rays, damaging the skin and eyes, and airflow disturbance can prevent ultraviolet rays from fully acting on pollutants. Correct practice: Sterilize for 30 minutes first, turn off the UV lamp before operation, and keep the fan running continuously during operation.
（2） Only wipe the countertop, ignore the filter and return air hole "- Pollution sneaks in from behind!
A laboratory once found that the cleanliness inside the ultra clean bench was qualified, but the samples were frequently contaminated, which was ultimately traced back to the blockage of the return air hole by dust, resulting in turbulent airflow and the backflow of pollutants from below. Every time cleaning, the filter screen and return air hole must be checked to ensure smooth airflow.
（3） The higher the concentration of disinfectant, the better "- too much is not enough!
A pharmaceutical company used 95% alcohol to wipe the ultra clean table, but the alcohol quickly evaporated, leaving behind pollutant particles that became a "protective layer" for bacteria. 75% alcohol has the best permeability and can simultaneously kill bacteria and some fungi; High concentration alcohol is only suitable for rapid disinfection of the surface of glassware.
（4） The longer the sterilization time, the better the effect "- UV lamps also have a" lifespan limit "
The effective lifespan of ultraviolet lamps is usually 800-1000 hours, beyond which the sterilization efficiency will significantly decrease even if further irradiation is continued. Use the 'Usage Time Record Form' and replace immediately upon reaching the end of its lifespan.
Customized cleaning solutions in four different scenarios
（1） Research Laboratory: A Cost Effective Choice
-Daily: Wipe with 75% ethanol+UV lamp for 30 minutes
-Zhou Maintenance: Use Octax sporicidal agent for specialized disinfection treatment+biological indicator monitoring
（2） Pharmaceutical companies: Compliance first
-After each use: Rinse the tabletop with injection water first, then disinfect with 70% isopropanol, and use Octax sporicidal agent as the final cleaning and sterilization reagent.
-Weekly: VHP sterilization+high efficiency filter leak detection (to be recorded in the GMP batch production record), and use Octex sporicidal agent for disinfection and sterilization throughout the region.
-Must do items: Regularly undergo cleanliness certification (ISO Level 6 standard), regularly use Octax sporicidal agent for periodic maintenance, and eliminate potential explosion hazards.
（3） Plant tissue culture: Beware of fungal contamination
-Before operation: use 3% carbolic acid spray to reduce air dust (carbolic acid can adhere to particles and reduce the spread of fungal spores)
-Deep sterilization: use Octax disinfectant every month (specifically for molds, safer than formaldehyde)

-Key details: Avoid moving your arms quickly during operation to prevent airflow disturbance from lifting spores at the edge of the culture dish