Does the use of the biosafety cabinet affect the results of the experiment? How to avoid the impact?

The use of biosafety cabinets may have an impact on experimental results, mainly because the air flow in the biosafety cabinet can affect parameters such as temperature, humidity, and air pressure in the experimental operation area, which may affect experimental results. In addition, if the filters, ultraviolet lights, fans, and other components inside the biosafety cabinet are not replaced or maintained in a timely manner, it may also lead to inaccurate experimental results.
To avoid these impacts, it is necessary to regularly check the normal operation of various components of the biosafety cabinet and follow the correct operating procedures and specifications, such as avoiding opening doors or placing large equipment inside the biosafety cabinet, to ensure that the air flow of the biosafety cabinet is not disturbed. In addition, the condition of the biosafety cabinet should be carefully checked before the experimental operation to ensure its normal operation. During the experimental operation, it is necessary to pay close attention to the temperature, humidity, air pressure and other parameters inside the biosafety cabinet to ensure the accuracy of the experimental results.
The biosafety cabinet is mainly used for handling biological hazardous substances, conducting biological experiments, and conducting operations such as pathogen reproduction, isolation, and cultivation. Specific experiments include but are not limited to the following categories:
Microbiological experiments: such as colony counting, isolation, identification, cultivation, etc.
Molecular biology experiments: such as DNA/RNA extraction, PCR, electrophoresis, etc.
Cell biology experiments: such as cell culture, cell separation, identification, culture, etc.
Immunological experiments: such as ELISA, immunofluorescence, immunoblotting, etc.
Animal experiments: such as animal reproduction, drug testing, etc.
It should be noted that the biosafety cabinet does not guarantee the accuracy of experimental results, but only provides a relatively safe experimental environment. The experimenter still needs to maintain a cautious attitude and strictly follow the experimental operating procedures for operation