How does the bacteria collector perform sterility test on liquid samples?

How does the bacteria collector perform sterility test on liquid samples?
It is 0.22 microporous membrane used to trap microorganisms in the test sample in the filter through the directional peristaltic pressurization principle of the bacteria collector μ M * 47mm or 0.45 μ On m * 47mm, the bacteria collector removes the antibacterial components by washing the filter membrane, and then directly introduces the required culture medium into the fully closed filter bacteria collector through the sampling pipe, and places it in the incubator for sterile culture.
How to perform sterility test on liquid samples with totally enclosed bacteria collector?
The totally enclosed bacteria collector is a constant speed peristaltic pump with the same frequency conversion control, which is used to safely and evenly transfer test articles of different packaging forms to the disposable sterile incubator device.
Instructions for sterility test of liquid sample with totally enclosed bacteria collector
1. Take out the incubator, check whether the package is intact, and open the plastic packaging bag in the sterile room.
2. Insert the incubators one by one on the stainless steel base.
3. Install the flexible hose of the bacteria collection incubator into the pump head of the intelligent bacteria collection instrument. The positioning is required to be accurate and the hose trend is smooth.
4. Open the cap pin hole of the sample to be tested and disinfect it. (If the ampoule sample is tested, first disinfect the neck of the ampoule, then open and disinfect the ampoule) Position the sample bottle.
5. Pull off the sheath of the double core syringe, insert it into the sample bottle, turn on the bacteria collector, and implement filtering and bacteria collection (avoid air intake of the double core syringe, and affect the air intake of the filter membrane due to the immersion of the drug solution).
6. After the completion of bacteria collection, if the sample contains bacteriostatic substance bacteria collector, the fully closed bacteria collector shall be properly cleaned with washing solution according to the requirements of the pharmacopoeia, and the cleaning method is the same as the bacteria collection process.
7. Disinfect the needle hole of the culture medium bottle.
8. Remove the rubber plug from the top air filter opening, put it on the bottom of the incubator, use the hose clamp to open and close the hose in turn, turn on the bacteria collector, and pump the culture medium into the incubator.
9. Clamp the hose connecting the incubator with clip, leave 5-6cm hose, cut off the rest, and insert the open end into the air filter opening.
10. Cultivate according to regulations.
11. Observe the culture condition with a totally closed bacteria collector. If it is necessary to take samples for isolation and culture, the hose can be disinfected, and the required amount can be extracted by inserting aseptic injection into the hose for further inspection.