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Planktonic bacteria sampler

日期:2022-09-25 04:40
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摘要:The planktonic bacteria sampler, also known as the air microbial sampler, is a highly efficient porous inhalation sampler. It is based on the Anderson impact principle with an impact velocity of 10.8m/s, which is equivalent to the fifth level of the Anderson impact level. The principle is that air is impacted to effectively collect particles in the air with a diameter ranging from 0.6 pm to 10lxm, and the collected particles in this range are directly impacted on the surface of the plate culture
The planktonic bacteria sampler, also known as the air microbial sampler, is a highly efficient porous inhalation sampler. It is based on the Anderson impact principle with an impact velocity of 10.8m/s, which is equivalent to the fifth level of the Anderson impact level. The principle is that air is impacted to effectively collect particles in the air with a diameter ranging from 0.6 pm to 10lxm, and the collected particles in this range are directly impacted on the surface of the plate culture medium along with the airflow.

Planktonic bacteria samplers are widely used


At present, the research on the types of planktonic bacteria samplers and sampling principles has been relatively mature. It can be roughly divided into three categories: microporous type, slit type and centrifugal type. Nowadays, planktonic bacteria sampling instruments have been widely used in the detection of air microorganisms in clean rooms and sterile environments such as pharmaceutical GMP factories, dairy products factories, hospital operating rooms, biological cleanliness, fermentation industries, and relevant research and teaching departments for air microorganism sampling research . Today, we mainly introduce the preparatory work and sampling steps of the planktonic bacteria sampler before sampling.
1. Planktonic bacteria sampler preparation work before sampling:
1. The temperature and humidity of the tested operating room (clean room) must meet the specified requirements; the static pressure difference, the number of air changes, and the air flow rate must be controlled within the specified values.
2. The one-way flow test should be started after the normal operation of the purification air-conditioning system is no less than 10 minutes; the non-unidirectional flow is no less than 30 minutes.
3. During the static test, there are no more than 2 people in the room. The tester should be on the leeward side of the sampling port during sampling.
4. The testing personnel should follow the aseptic operation procedures. Take all measures to prevent contamination of the sampling port and other man-made contamination of the sample.
5. Test personnel must wear clean clothes that meet the cleanliness level.
6. Before the planktonic bacteria test, the tested operating room (clean room) has been disinfected.
2. Sampling steps of the planktonic bacteria sampler:
1. Prepare a petri dish in a clean room with a platform (XX clean room, XX measuring point), and wipe the platform with medical degreased cotton soaked in 75% ethanol before operation. The hands of the tester are strictly disinfected in the same way. In the preparation process, carefully check the quality of the culture medium again. If it is deteriorated, damaged or contaminated, it cannot be used.
2. Wipe the sampling port of the sampler, the sampling tube, the cover of the sampler, the inner and outer surfaces of the turntable and the cover with medical degreased cotton soaked in 75% ethanol.
3. After the sampler is disinfected, do not put in the petri dish, start the instrument idling for no less than 5 minutes to evaporate the residual disinfectant in the instrument.
4. Set the sampling volume according to the cleanliness level of the operating room, the flow rate of the sampler (L/min) X sampling time (min) = sampling volume (L)
5. Put in the petri dish, close the lid and adjust the gap height of the sampler. During the sampling operation, the petri dish should be handled gently, and the outer diameter of the petri dish should be grasped with "five fingers" to try to avoid artificial contamination of the sample.
6. The layout of sampling points and the number of sampling points.